Patterned cell arrays and patterned co-cultures on polydopamine-modified poly(vinyl alcohol) hydrogels

Protein-patterned hydrogels: Customized cell microenvironments.

Patterned metal nanowire arrays from photolithographically-modified templates.

An effective procedure for the fabrication of patterned nanowire arrays with micron-sized features is presented. Photolithographic methods are utilized to form set patterns onto porous anodic alumina membranes (AAM), and these modified membranes can then be used in the electrochemical growth of nanowire arrays. This approach readily allows the formation of a variety of nanowire array patterns w...

Magnetically and biologically active bead-patterned hydrogels.

We present a new approach to the direct patterning of biologically and magnetically active microbeads in nonbiofouling polymer scaffolds for use in microfluidic devices. Briefly, the process involves treatment of a glass substrate, conformal contact bonding of a PDMS microchannel on the substrate, filling of the channel with beads and prepolymer solution, and UV-initiated photopolymerization of...

Thermal stability of patterned Co/Pd nanodot arrays

We have studied the magnetic reversal and thermal stability of [Co(0.3 nm)/Pd(0.7 nm)]N multilayers patterned into 35-nm-diameter nanodot arrays. The short-time coercive fields are relatively constant with N while the room-temperature thermal stability parameter increases nearly linearly with N. However the magnetic switching volume extracted from the thermal stability is significantly less tha...

Micropatterned Co-cultures of Human Embryonic Stem Cell with Murine Embryonic Fibroblasts on Microwell Patterned Substrates

Human embryonic stem (hES) cells are generally cultured on top of a feeder layer formed by mitotically inactivated murine embryonic fibroblasts (MEFs) to maintain their undifferentiated state. The culture of hES cells in this co-culture system presents several challenges since it is difficult to control cell cluster size. Large cell clusters tend to differentiate at the borders, and clusters wi...